ProtoxHysan - Application

Mould Growth

If there is discoloration and growth of mould, disinfect or otherwise wash off the affected surface with ProtoxHysan. Possible combined with vacuum cleaning, ice cleaning,  mechanical cleaning, dry steam cleaning or similar before treatment.

In the indoor environment, it should always be endeavored that a surface attacked by moulds be cleaned to a level of purity, similar to surfaces not attacked by moulds.


ProtoxHysan is applied with a garden- or low pressure sprayer, after which the surface is scrubbed with a brush, sponge or similar. ProtoxHysan can also be laid out with a fogger to remove odours in difficult-to-reach rooms.

Indoor climate:

With ProtoxHysan, there is no limitation on the size of the treated area, as no chemical residues are left behind in the building, cf indoor climate report.

Course of action:


Mould may typically be known to be black-brown, blue-green and whitish pests and stains on the affected building part. Often the mould sits on the inside of the wallpaper, but it can also sit behind the wallpaper and even behind the plaster on eg. bricks, concrete, etc.

If the mould is behind the wallpaper and or the plaster layer, you MUST REMOVE the wallpaper or the plaster BEFORE you start cleaning the mould


  • Garden- or low pressure-spray
  • Protective glowes
  • Nylon brush
  • Possible cover plastic
  • Waste wiping cloths
  • Possible respirator
  • ProClean quality control (or other test)


ProtoxHysan is a concentrate that can be diluted as needed. *

Concentrated ProtoxHysan - for particularly stubborn discolouration

1 part ProtoxHysan + 1 part water - severe attacks

1 part ProtoxHysan + 2 parts water - medium severe attacks

1 part ProtoxHysan + 3 parts water - light attack

Cleaning with ProtoxHysan:

  1. The surface is thoroughly vacuum cleaned with a vacuum cleaner with HEPA filter.
  2. A solution of ProtoxHysan and water is mixed in a garden- or low pressure-spray.
  3. The moulded surface is sprayed onto the ProtoxHysan solution and the surface is thoroughly cleaned with a nylon brush until the mould attack is released from the surface.. Consumption approx. 1/2 litre/m².
  4. The cleaned surface is now rinsed clean with the solution from the garden- low pressure-spray. This removes as much as possible of spores, particles and dirt from the surface.
  5. The "wet" Hysan-treated surface should now stand for up to 1 day and act on the surface, thereby oxygenating(oxidizing) residual odour. 
  6. After 1 day´s working time, the surface may possible be wiped clean with clean water, but this is usually not necessary.
  7. If there is a risk of regrowth due to persistent moisture in the substrate, the surface can be treated afterwards with ProtoxProtect.

Respiratory protection:

If the mould remediation task is of a greater extent (ie, the area occupies more than a few A4 pages, and especially if the task involves removal of moulded materials (wallpaper, plaster, and / or sanding, insulation, etc.), there is a risk that dust and mould spores can be released to the air. It is therefore recommended to use a respirator - minimum of type P3. A typical combination respirator type A2 / B2 / P3 protects against inhaling particles from the moulds and at the same time against the inhalation of aerosols from the disinfectant when on spraying on ProtoxHysan.

Quality control:

If you want to check if the mould has been removed, you can do so with a ProClean test. The ProClean test is a standard protein test that shows whether the mould proteins have been removed.

Using ProClean

The ProClean gives a clear violet colour reaction if foreign proteins (protein) are present from the mould. Testing can be performed already 10-15 minutes after washing has been done with ProtoxHysan. The ProClean cotton swab is pulled out of the plastic sheath and the surface (10x10 cm) is swabbed - if swab becomes dirty, cleaning is not sufficiently thorough - clean again and repeat swab. The swab is placed back in the holster and the pipette at the top is broken and the liquid is pressed down over the swab. Shake gently. Color reaction should be read after 5 minutes - but within 10 minutes. If the area is cleaned well enough, a clear light green color is seen. However, if the area is contaminated, the liquid becomes violet.


NOTE! - It is important that the area is rinsed/wiped thoroughly so that dead particles are removed as much as possible. These cannot be measured with ProClean.

Odour removal:

Bad odour from mould growth, bacterial growth, dead animals, corpses etc. can be removed by soaking / washing the affected surfaces or mist spray with ProtoxHysan. It can be combined with the ProtoxAbScent bag, which is suspended for residual odour removal.

Mould Prevention

It is important to remove the cause of the mould attack. If the cause is not removed, the mould will most likely reappear. If the cause cannot be eliminated, it is possible to prevent new mould attacks with ProtoxProtect, which is applied to the cleaned surface by brush, spraying or similar.

Cleaning Ducts

Used as a fogging, ProtoxHysan is particularly suitable for disinfecting ventilation systems, possibly after prior mechanical cleaning.