MANUAL for using HYSAN and BIOX for mold removal
Contact: Protox – phone 75504022
CLEANING OF MOLDS, BACTERIA, etc. with HYSAN AND BIOX
- Why HYSAN or BIOX ?
Both products are indoor climate neutral and therefore release no substances into the indoor air after drying, unlike most other disinfectants.
What should I use where?
| HYSAN | BIOX |
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PREPARATION:
Remove all infested building waste, insulation, wall/concrete remains, dirt, etc. Ensure VERY thorough clean-up before initiating cleansing.
REMEMBER – mold removal is the last process in the remediation process - i.e. NO other craftsmen drilling holes, breaking down, moving building materials around, etc., while you are carrying out mold removal or after.
ATTENTION!
In case of deeper mold attack in e.g. plaster wall or wood, it may be necessary to completely REMOVE the affected surface, as the mold has grown into the surface. Otherwise, you run the risk of "pulling mold out of the wall" during cleaning and having the work discarded. Agree if necessary with responsible ADVISOR which cleaninglevel to be achieved! This MUST always be tested before starting.
HYSAN treatment:
METHOD TESTING:
For larger cleaning tasks, a small test area is selected where you test the cleaning method you want to use. For example 3 fields of 20×20 cm are made which are cleaned with HYSAN diluted 1:1, 1:2 and 1:3. Possibly. a half-activated HYSAN (contact PROTOX) is also tested.
Based on a quality control with ProClean (quick test) or other method, the most effective dilution is selected. Agree if necessary with ADVISOR before cleaning is initiated.
HOW IS HYSAN DILUTED?
| Concentrated HYSAN | – for particularly stubborn discolouration |
| 1:1 – 1 part HYSAN + 1 part water | – for heavy attacks |
| 1:2 – 1 part HYSAN + 2 parts water | – medium attacks |
| 1:3 – 1 part HYSAN + 3 parts water | – light attacks |
HOW IS HYSAN ACTIVATED?
25 ml is added. activator for 5 liters of HYSAN the day before use - NOTE! – activation entails 100% personal protection as for BIOX-treatment !
TOOLS:
Garden sprayer
Protective gloves
Brush – of the semi-rigid nylon type
Cover
Cloths for wiping up spills
Ventilation/ air cleaner (?)
Respiratory protection – B2/P3
ProClean quality control cotton swabs (possibly another test)
STANDARD CLEANING with HYSAN:
When removing mold, it is important that before bigger work is initiated, conduct a test of which concentration of HYSAN works best on the mold-infested surface and material type. Cleaning with HYSAN can be combined with mechanical cleaning as needed, e.g. degreasing, de-icing, high-pressure cleaning with hot water, etc. The most used method is described in the following:
- The surface is vacuumed thoroughly with HEPA
- A solution of HYSAN and water – e.g. 1 part HYSAN + 1-2 parts water - mixed in a 5 liter garden sprayer (Gloria, Ginge etc.)
- The mold-infested surface is sprayed with the HYSAN solution and the surface is worked thoroughly with a nylon brush until the mold infestation is loosened from the surface. Consumption approx. 1/2 liter per m2.
- The processed surface is now rinsed clean with the HYSAN solution from the garden sprayer. This removes as much as possible of spores, particles and dirt from the surface.
- The "wet" Hysan-treated surface should now stand for up to 1 day and work in the surface, whereby residual odors are oxygenated (oxidized).
- After 1 day's working time, the surface can be wiped with clean water.
- If there is a risk of regrowth due to persistent moisture in the substrate, it can be treated with
OWN QUALITY CONTROL:
ProClean – if the cotton swab gets dirty – all over again !
ProClean test approx. 5 minutes after washing!
ProClean – The surface (approx. 5×5 cm) is wiped with the cotton swab. The swab is put back in the case, after which the pipette at the top is broken and the liquid is pressed down over the swab. Shake gently. Color reaction is read after 10 minutes. Should give clear green color reaction. Use a reasonable number of samples per area so that the best possible safety is achieved.
See appendix 1 for further information on analysis methods
LOG BOOK
For major tasks, a logbook must always be kept in which significant details about the execution of the task, including checks, are written down. For example dilutions, special conditions, exact location and times for both internal and external quality control.
FOG SPRAYING FOR ODOR REMOVAL
HYSAN is used by mist spraying to both disinfect and remove bad odors from mold growth, soot damage after a fire, bacterial growth, urine, faeces, dead animals/people etc. See also AbScent deodorizer. Suitable fog spraying equipment is used, such as B&G©, Wanjet© or similar. The mist sprayer is filled with HYSAN, concentrated or diluted 1:1. The room to be treated, e.g. crawl space, closed cavities, living spaces, etc. filled with the HYSAN mist and the room sealed. 1-3 days later, the room is thoroughly ventilated and the effect assessed.
Fog spraying should generally not be carried out without prior mechanical cleaning of the affected surfaces.
CLEANING OF VENTILATION DUCTS
Used as a mist spray, HYSAN is particularly suitable for disinfecting ventilation systems, possibly after previous mechanical cleaning.
EDUCATION
None required.
SECURITY
Read the safety data sheet before use. For fog spraying, a full face mask with a B2/P2 filter must be used. Do not expose to temp. above 50 degrees Celsius.
PREVENTION
If there is a need to prevent future mold attacks, you should subsequently use PROTOXSKIMMEL.
EXTERNAL CONTROL
External quality control is carried out after approx. 1 day.
VENTILATION
Ensure good ventilation during the treatment and during the drying period.
SPENDING
Approx. 1/2 liter of working solution per m2 surface depending on the nature and absorbency of the surface.
CORROSION
HYSAN can cause corrosion on certain metals at higher concentrations. Therefore, these should be protected by fog spraying in particular.
STORAGE
Must be done dark and cool. Do not store together with food.
ANNEX 1
A little about quality control
There are various methods of quality control. The following describes some of the typically used methods:
MycoMeter is a chemical test that is carried out with a cotton swab and which measures some chemical components in the mold. If this test is used, it is important that the surface is rinsed very thoroughly after treatment, as dead mold particles that may remain on the surface will otherwise affect the test. For a measurement number of more than approx. 25, the purification will often be discarded. If HYSAN is used correctly, it will not normally be a problem to achieve a reading of less than 25. The test does not tell anything about which molds are present.
Pro-Clean is a new method which easily shows the presence of protein on an attacked surface. Thus, molds contain proteins which, among other things, are the substances known to cause allergic reactions in people who are sensitive to mould. Here, too, you have to be thorough with the cleaning, but Hysan is effective in denaturing the foreign proteins.
Contact imprint is a widely used method when you want to determine by species which molds are present. However, it is necessary to grow the impressions in the laboratory, which is why a response time of 4-7 days must be expected. Contact impressions tell whether the mold is dead and not necessarily whether it has been removed. In the indoor environment, it should always be aimed that a surface infested by mold is cleaned to a level of cleanliness that at least corresponds to that found on surfaces not infested by mold - i.e. low growth – max. 10 colonies by impression on contact impression.
Tape impressions/material samples makes it possible to determine the presence of mold directly under a microscope, including whether it has been removed during cleaning.
Regardless of which control methods are used, it is important to be very careful when carrying out the work. Remember – it is not enough to simply dry out and/or etc. kill the mold growth. As a rule, it must also be removed, as both dead and live molds contain the same allergenic substances.
BIOX treatment:
BIOX can only be used activated.
HOW IS BIOX ACTIVATED?
40 ml is added. activator for 5 ltr. BIOX min. 24 hours before use. It activated BIOX should take on a lemon yellow color 24 hours after activation and before use.
TOOLS:
Sweeping diet.
Water jug with spreader spout/boom.
Protective gloves
Protective suit
Covering of metals.
Powerful fan, Ø=250 or more.
Chlorine dioxide measures an advantage (safety equipment)
Respiratory protection – B2/P3 with eye protection, preferably air supply.
ProClean quality control cotton swabs (possibly another test)
STANDARD CLEANING with BIOX:
PREPARATION:
– see previous description on page 1. Ventilation systems are prepared in such a way that exhaust air is led out into the open where there is no risk of unauthorized persons coming into contact with the exhaust air. Wind direction must be taken into account. It would be best if e.g. a flex hose on the ventilator which can be moved around during the treatment.
BIOX treatment is best performed by 2 people. One performs the application and the other processes the BIOX on the surface.
- Thorough vacuuming with HEPA
- The activated BIOX is poured into a jug of water. The BIOX is slowly watered out onto the concrete deck with approx. 0.5-1 litre/m2. With the broom, the liquid is "pushed" around with calm movements and the BIOX MUST be kept on the surface at a minimum 60 seconds. It is important not to "scrub" with the brush, as the BIOX thereby loses its effect.
- Application is carried out continuously by one person as the other person processes the BIOX until the entire surface is treated.
- As BIOX is watered out on the surface and you work your way through the room, the ventilation MOVES along to remove as much excess BIOX outgassing as
- The course therefore takes 1 minute/m2 – or approx. 1 hour per 60 m2.
- When most of the chlorine dioxide degassing has subsided after 15-30 minutes, a control measurement is made with the chlorine dioxide meter at floor level. Only when this shows a level below 0.1 ppm respirators must be removed and users of the room can move around the room again.
- Excess liquid on the concrete deck is dried up or allowed to evaporate. Possibly. is used
EDUCATION
May only be used by trained personnel.
SECURITY
Read the safety data sheet before use. A full mask with a B2/P3 filter must be used as a minimum. Do not expose to temp. above 50 degrees Celsius. When working in basements, i.e. below ground level, an external air supply must be used, as chlorine dioxide is heavier than air and will thereby be able to condense the oxygen. Employees who are hypersensitive to chlorine must not carry out the treatment.
PREVENTION
If there is a need to prevent future mold attacks, you should subsequently use PROTOXSKIMMEL.
CONTROL
Quality control is performed immediately post processing. You can use, for example, ProClean as self-control. Other control methods can be used as external control.
VENTILATION
Ensure good ventilation during the treatment and during the drying period. Forced ventilation must be established during the execution of the treatment, cf. above.
DURABILITY
Approx. 3-4 weeks after activation
CORROSION
BIOX can cause corrosion on certain metals. Therefore, these should be protected during treatment (e.g. hood covers, coat racks, lamps, electronics etc.)
STORAGE
Must be served dark and cool with a tightly screwed lid.