MANUAL for the use of HYSAN and BIOX in mould remediation
Contact: Protox - phone 75504022
Removing mould, bacteria, etc. with HYSAN and BIOX
- What's the point? HYSAN or BIOX ?
Both products are indoor climate neutral and therefore do not release any substances into the indoor air after drying, unlike most other disinfectants.
What should I use where?
| HYSAN | BIOX |
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PREPARATIONS:
Remove all infested building waste, insulation, masonry/concrete debris, dirt, etc. Make sure to clean up VERY carefully before starting the cleaning process.
REMEMBER - mould removal is the last process in the remediation process - NO other tradesmen drilling holes, breaking down, moving building materials around, etc. while performing mould remediation or afterwards.
ATTENTION!
For deeper mould infestations in e.g. plaster walls or wood. plaster wall or wood, it may be necessary to completely REMOVE the affected surface as the mould has grown into the surface. Otherwise you risk "pulling mould out of the wall" during cleaning and having the work rejected. If necessary, agree with the responsible CONSULTANT which cleanlinesslevel to be achieved! This MUST always be tested before commissioning.
HYSAN treatment:
TESTING METHODOLOGY:
For larger cleaning jobs, a small test area is selected to test the cleaning method you want to use. For example. 3 fields of 20×20 cm are made and cleaned with HYSAN diluted 1:1, 1:2 and 1:3. You can also test a semi-activated HYSAN (contact PROTOX).
Based on a quality check with ProClean (quick test) or other method, choose the most effective dilution. If necessary, consult with a CONSULTANT before cleaning is initiated.
HOW IS HYSAN DILUTED?
| Concentrated HYSAN | - for particularly stubborn discolouration |
| 1:1 - 1 part HYSAN + 1 part water | - For heavy attacks |
| 1:2 - 1 part HYSAN + 2 parts water | - medium attacks |
| 1:3 - 1 part HYSAN + 3 parts water | - light attacks |
HOW IS HYSAN ACTIVATED?
Add 25 ml of activator to 5 litres. HYSAN the day before use - Attention! - activation 100% provides personal protection as for BIOX-treatment !
EDITORS:
Garden sprayer
Protective gloves
Brush - semi-rigid nylon type
Hedging
Wipes for wiping up spillages
Ventilation/ air purifier (?)
Respiratory protection - B2/P3
ProClean Quality control cotton buds (possibly another test)
STANDARD CLEANING with HYSAN:
When cleaning mould, it is important that before bigger When starting work, test which concentration of HYSAN works best on the mould-affected surface and type of material. Cleaning with HYSAN can be combined with mechanical cleaning as needed, e.g. grinding, ice removal, high-pressure cleaning with hot water, etc. The most commonly used method is described in the following:
- Vacuuming the surface thoroughly with HEPA
- A solution of HYSAN and water - e.g. 1 part HYSAN + 1-2 parts water - is mixed in a 5 litre garden sprayer (Gloria, Ginge or similar)
- Spray the mould-affected surface with the HYSAN solution and work the surface thoroughly with a nylon brush until the mould is removed loosened from the surface. Consumption approx. 1/2 litre per m2.
- The treated surface is now rinsed clean with the HYSAN solution from the garden sprayer. This removes as many spores, particles and dirt from the surface as possible.
- The "wet" Hysan-treated surface should now be left for up to 1 day to act on the surface, whereby residual odours are oxygenated (oxidised).
- After 1 day, the surface can be wiped with clean water.
- If there is a risk of regrowth due to persistent moisture in the substrate, you can re-treat with
OWN - QUALITY CONTROL:
ProClean - if the cotton swab gets dirty all over again!
ProClean test approx. 5 minutes after washing!
ProClean - Swab the surface (approx. 5×5 cm) with the swab. Place the swab back into the casing, break the pipette at the top and press the liquid down over the swab. Shake gently. Read the colour reaction after 10 minutes. Should give a clear green colour reaction. Use a reasonable number of samples per area to maximise safety.
See appendix 1 for more info on analysis methods
LOGBOOK
For larger tasks, a logbook must always be kept where essential details about the task's execution, including checks, are recorded. For example. dilutions, special conditions, exact location and times of both internal and external quality control.
MIST SPRAYING FOR ODOUR REMOVAL
HYSAN is used in mist spraying to both disinfect and remove bad odours from mould growth, soot damage after fire, bacterial growth, urine, faecal matter, dead animals/humans etc. See also AbScent odour remover. Suitable mist spraying equipment is used, such as B&G©, Wanjet© or similar. Fill the mist sprayer with HYSAN, concentrated or diluted 1:1. The room to be treated, e.g. The room to be treated, e.g. crawl spaces, closed cavities, living spaces, etc. is filled with the HYSAN mist and the room is sealed. 1-3 days later, ventilate the room thoroughly and assess the effect.
Fog spraying should generally not be carried out without prior mechanical cleaning of the affected surfaces.
CLEANING OF VENTILATION DUCTS
When used as a mist spray, HYSAN is ideal for disinfecting ventilation systems, possibly after prior mechanical cleaning.
EDUCATION
None required.
SAFETY
Read safety data sheet before use. For mist spraying, use full face mask with B2/P2 filter. Do not expose to temp. above 50 degrees centigrade.
PREVENTION
If there is a need to prevent future mould infestations, PROTOXSKIMMEL should be used afterwards.
EXTERNAL CONTROL
External quality control is performed after approximately 1 day.
VENTILATION
Ensure good ventilation during treatment and during the drying period.
CONSUMPTION
Approx. 1/2 litre of application solution per m2 of surface, depending on the nature and absorbency of the surface.
CORROSION
HYSAN can cause corrosion on certain metals at higher concentrations. Therefore, these should be protected, especially by mist spraying.
STORAGE
Store in a dark and cool place. Do not store with foodstuffs.
ANNEX 1
A little about quality control
There are different methods for quality control. Some of the most commonly used methods are described below:
MycoMeter is a chemical test performed with a cotton swab that measures some chemical constituents of the mould. When using this test, it is important that the surface is rinsed very thoroughly after treatment, as any dead mould particles remaining on the surface will otherwise show up on the test. With a measurement number of more than about 25, the cleaning solution will often be discarded. If HYSAN is used correctly, it will normally not be a problem to achieve a reading below 25. The test does not tell you anything about which moulds are present.
Pro-Clean is a new method that easily shows the presence of protein on an infested surface. Moulds contain proteins that are known to cause allergic reactions in mould-sensitive people. Here, too, you need to be thorough with cleaning, but Hysan is effective at denaturing the foreign proteins.
Contact print is a widely used method when you want to determine which moulds are present. However, it is necessary to grow the prints in the laboratory, so a response time of 4-7 days should be expected. Contact prints indicate whether the mould is dead and not necessarily whether it has been removed. In the indoor environment, it should always be ensured that a mould-affected surface is cleaned to a level of cleanliness at least equivalent to that found on non-mould-affected surfaces - i.e. poor growth - max. 10 colonies on contact prints.
Tape impressions/material samples makes it possible to determine the presence of mould directly under a microscope, including whether it has been removed during cleaning.
Regardless of which control methods are used, it is important to be very careful when carrying out the work. Remember - it is not enough to simply dry out and/or kill the mould growth. It must also be removed as both dead and living mould contains the same allergenic substances.
BIOX treatment:
BIOX can only be used activated.
HOW IS BIOX ACTIVATED?
Add 40 ml activator to 5 litres. BIOX min. 24 hours before use. The activated BIOX should take on a lemon yellow colour 24 hours after activation and before use.
EDITORS:
Sweep broom.
Watering can with spout/boom.
Protective gloves
Protective suit
Uncovering metals.
Powerful fan, Ø=250 or more. more.
Chlorine dioxide meter an advantage (safety equipment)
Respiratory protection - B2/P3 with eye protection, preferably air supply.
ProClean Quality control cotton buds (possibly another test)
STANDARD CLEANING with BIOX:
PREPARATION:
– see previous description on page 1. Ventilation systems must be prepared so that exhaust air is led out into the open air where there is no risk of unauthorised persons coming into contact with the exhaust air. Wind direction must be taken into account. It would be best if the fan is fitted with e.g. a flexible hose on the fan that can be moved around during treatment.
BIOX treatment is best done by 2 people. One does the application and the other works on the surface of the BIOX.
- Vacuuming thoroughly with HEPA
- Pour the activated BIOX into a watering can. Slowly water the BIOX onto the concrete slab at approx. 0.5-1 litre/m2. The broom "pushes" the liquid around with gentle movements and the BIOX MUST be kept on the surface for at least 60 seconds. It is important not to "scrub" with the brush, as the BIOX will lose its effect.
- Application is carried out continuously by one person as the other person processes the BIOX until the entire surface is treated.
- As BIOX is watered out on the surface and you work your way through the room, the ventilation is MOVED to remove as much excess BIOX degassing as possible
- The process therefore takes 1 minute/m2 - or approx. 1 hour per 60 m2.
- After 15-30 minutes, when most chlorine dioxide outgassing has subsided, take a control measurement with the chlorine dioxide meter at floor level. Only when this shows a level below 0.1 ppm Respirators must be removed and room users can move around the room again.
- Excess liquid on the concrete deck is dried up or allowed to evaporate. If necessary, use
EDUCATION
Use only by trained personnel.
SAFETY
Read safety data sheet before use. Use a full face mask with B2/P3 filter as a minimum. Do not expose to temp. above 50 degrees centigrade. When working in basements, i.e. below ground level, an external air supply must be used as chlorine dioxide is heavier than air and can vapourise oxygen. Employees who are hypersensitive to chlorine must not perform the treatment.
PREVENTION
If there is a need to prevent future mould infestations, PROTOXSKIMMEL should be used afterwards.
CONTROL
Quality control is carried out Directly after treatment. For example, the following can be used. ProClean as self-checking. Other control methods can be used as external control.
VENTILATION
Ensure good ventilation during treatment and during the drying period. Forced ventilation must be established during the treatment as described above.
DURABILITY
Approximately 3-4 weeks after activation
CORROSION
BIOX can cause corrosion on certain metals. Therefore, these should be protected during treatment (e.g. cooker hood covers, coat hooks, lamps, electronics, etc.).
STORAGE
Store in a dark and cool place with a tightly screwed lid.